Microglia are scavengers of the brain environment that clear dead cells, debris, and microbes. In Alzheimer’s disease, microglia get activated to phagocytose damaged neurons, extracellular Amyoid-β, and Tau deposits. Several Tau internalization mechanisms of microglia have been studied which include phagocytosis, pinocytosis, and receptor-mediated endocytosis. In this chapter, we have visualized microglial phagocytic structures that are actin-rich cup-like extensions, which surrounds extracellular Tau species by wide-field fluorescence and confocal microscopy. We have shown the association of filamentous actin in Tau phagocytosis along the assembly of LC-3 molecules to phagosomes. The 3-dimensional, orthogonal and gallery wise representation of these phagocytic structures provides an overview of the phagocytic mechanism of extracellular Tau by microglia.
CITATION STYLE
Chidambaram, H., Desale, S. E., Qureshi, T., & Chinnathambi, S. (2024). Microglial Uptake of Extracellular Tau by Actin-Mediated Phagocytosis. In Methods in Molecular Biology (Vol. 2761, pp. 231–243). Humana Press Inc. https://doi.org/10.1007/978-1-0716-3662-6_16
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