Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these interactions due to its speed, high efficiency, and low sample/reagent consumption. In addition, CE works effectively under a wide range of physiologically relevant conditions. This chapter presents state-of-the-art on CE methods for studying GAG–protein interactions including affinity capillary electrophoresis (ACE), capillary zone electrophoresis (CZE), frontal analysis (FA)/frontal analysis continuous capillary electrophoresis (FACCE), and capillary electrokinetic chromatography (CEC) with detailed experimental protocols for ACE and CZE methods.
CITATION STYLE
Liang, A., & Desai, U. R. (2015). Studying Glycosaminoglycan–Protein Interactions Using Capillary Electrophoresis. Methods in Molecular Biology, 1229, 355–375. https://doi.org/10.1007/978-1-4939-1714-3_28
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