Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation

Abstract

Background: Glycosaminoglycans influence stem cell fate but their combination with biomaterials remains to be optimized. Results: GAG bound to scaffolds presented essential sulfation epitopes and proved biologically active. Conclusion: Use of plasma polymerized allylamine proved effective in functionalizing a fibrous extracellular matrix mimic. Significance: The biomaterial has broad applicability to stem cell culture and has potential future applications in regenerative medicine. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.