Design and evaluation of the antimicrobial properties of ackee seed extract silver nanoparticle film formulations

Abstract

BACKGROUND: Plant-extract-reduced metal nanoparticles provide means of overcoming microbial resistance. Incorporating them into appropriate pharmaceutical formulations will enhance their portability and ease of administration. OBJECTIVES: To synthesize silver nanoparticles using methanol extracts of the seeds of Blighia sapida as capping agents and formulating the products in antimicrobial films. MATERIAL AND METHODS: Phytochemical screening of the methanol extract of Blighia sapida K.D. Koenig (ackee) seeds was performed and its antioxidant properties were determined using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. The green synthesis of ackee seed extract silver nanoparticles (ASAgNPs) was accomplished with reacting 1 mM of aqueous silver nitrate (AgNO3) and the methanol extract in a flask; the bioreduction was performed at 37°C for 72 h. The resulting nanoparticles were lyophilized and characterized using UV-visible spectrophotometry, Fourier-transform infrared spectroscopy (FTIR) and photomicrography. The nanoparticles were further formulated into films using starch and carboxymethyl cellulose using the solvent evaporation method. The extract, biosynthesized nanoparticles and film formulations were screened for antimicrobial activity against several pathogens using the agar well diffusion method. RESULTS: The methanol seed extracts of the ackee fruit contained saponins, tannins, flavonoids, terpenoids, and anthraquinones. The extract exhibited significant antioxidant properties. The nanoparticles and film formulations had a broader range of activity against microbes than the plant extract, exhibiting significant activity against Escherichia coli ATCC 700728, Salmonella typhi ATCC 14028, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853. Activity was also observed with Candida krusei, C. albicans, and Penicillium sp. It is noteworthy that this last organism showed resistance to fluconazole. CONCLUSIONS: Ackee seed extract silver nanoparticles exhibited a synergistic antimicrobial activity against several pathogens. Film formulations of the nanoparticles retained this antimicrobial activity and allowed the product to be presented in a consumer-ready form.