An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways. © 2011 Wang et al; licensee BioMed Central Ltd.
CITATION STYLE
Wang, X., Dong, Y., Jiwani, A. J., Zou, Y., Pastor, J., Kuro-O, M., … Yang, C. R. (2011). Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions. Proteome Science, 9. https://doi.org/10.1186/1477-5956-9-53
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