Larvicidal effects of supercritical extract of Melia azedarach and Bacillus thuringiensis var. israelensis product on late instar of Aedes Aegypti (Diptera: Culicidae)

Abstract

The intense use of synthetic products for the control of Aedes aegypti has led to the development of resistance and imposes health risks to other flora and fauna. This study assessed the larvicidal potential of Melia azedarach (Meliaceae) fruit extract obtained by the supercritical carbon dioxide extraction method and Bacillus thuringiensis var. israelensis (Bti) on second and third instar A. aegypti larvae under laboratory conditions. The assays were performed in disposable cups containing 100 250, 500, 750, 1,000 and 2,000 μg/ml extracts of M. azedarach, and 0.0005, 0.001, 0.0015, 0.002, and 0.0025 g/L of VectoBac WG®; the controls contained only water. Ten active larvae were added to each well, and the number of living larvae was counted each day for 72 h. Treatment efficiency was calculated using Abbott’s equation. Insecticidal efficiency increased with M. azedarach extract concentration, with significant effects in the 1,000 and 2,000 μg/mL concentration range. Bti efficiency was 100 % in the first 24 h. The results indicate the possibility of A. aegypti population control using supercritical extracts of M. azedarach.