Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health

Abstract

Emerging antimicrobial resistance among CNS is a concern in veterinary and human medicine. Coagulase test is considered as the key test to differentiate staphylococci to two groups, coagulase positive staphylococci (CPS) and coagulase negative staphylococci (CNS). A total of 200 Staphylococci strains were isolated with percentage 66.7% (200/300) from quarter milk samples. The total of S. aureus strains are 70 with percentage 35% (70/200). Among 70 strains of S. aureus, 30 strains are coagulase positive S. aureus with percentage 43% (30/70) and coagulase negative S. aureus 57% (40/70). CNS other than S. aureus was detected with percentage 65% (130/200) from subclinical mastitic cows. We examine sixty isolates of staphylococci recovered from subclinical mastitis in dairy cattle which divided as ten isolates of coagulase positive S. aureus (CP S. aureus), ten isolates of coagulase negative S. aureus (CN S. aureus) and forty isolates of coagulase negative staphylococci (CNS) which identified using API-Staph Kits as S. chromogenes, S. simulans, S. haemolyticus, S. epidermidis and S. cohnii. The genotypic detection of coa gene and mecA gene was screened in CP S. aureus, CN S. aureus and CNS.