Calcium and calcium-binding proteins play crucial roles in the regulation of actin dynamics, which contributes to cancer cell migration and invasion. In this chapter, we have focused on a three-dimensional imaging method to explore the pathophysiological function of EF-hand domain-containing protein D2 (EFHD2), a novel actin-binding protein. To overcome the limitations of two-dimensional imaging on substrate-coated cover glass for examination of invasive protrusions of cancer cells, we suggest three-dimensional reconstruction from optical z-sections of cells cultured on substrate-impregnated membrane filters of Transwell.
CITATION STYLE
Huh, Y. H., Yeo, Y. R., & Song, W. K. (2019). Three-dimensional reconstruction imaging method to study the function of EFHD2 in invadopodia formation. In Methods in Molecular Biology (Vol. 1929, pp. 607–613). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9030-6_38
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