importance of the sterilization procedure for producing vigorous cherry plants (Prunus sp.) in vitro

Abstract

The micropropagation of woody species is a good method for producing juvenile plant material in the short time. The sterilization procedure can have a slightly negative influence on the inoculation of winter buds, the induction and continuous growth of cherry microcuttings. The surface sterilization with the dicloroisocyanuric acid Na2 salt (DICA) was carried out in two procedures. In the procedure 1, 70% ethanol for 30 sec and 20 g/l of DICA for 20 min were used, while in the procedure 2 only 16.6 g/l of DICA for 15 min was used. Compared with the procedure 1, the procedure 2 showed significantly (P £ 0.05) better induction results and the production of the most vigorous plants (better growth, better root formation).