Cell growth and Trametes versicolor laccase production in transformed Pichia pastoris cultured by solid-state or submerged fermentations

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Abstract

BACKGROUND: Growth kinetics of Pichia pastoris and heterologous expression of Trametes versicolor laccase were compared. This is the first study of its kind between solid-state yeast cultures done on polyurethane foam (PUF) and submerged liquid fermentations (SmF). RESULTS: The maximum values of biomass were similar for SSF (solid-state fermentation) and SmF experiments when the BOD (biochemical oxygen demand) was lower than 100 g L-1. For higher BOD levels, the maximum values of biomass were 55 g L-1 (SSF) and 35 g L-1 (SmF). Micrographs of PUF preparations showed yeast growing within liquid lamellae, thinner than 100 μm, forming large horizontal aggregates. Yeast aggregates were much smaller in SmF than in SSF experiments; however, laccase expression was lower in PUF than in SmF, unless the methanol concentration was increased to 63 g L-1, which was inhibitory only to the SmF system. CONCLUSION: The results show that oxygen mass transfer is more efficient in SSF, which is related to the higher area/volume ratio compared with SmF. Induction differences may also be due to hindered diffusion of methanol within large yeast aggregates. © 2009 Society of Chemical Industry.

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López, M., Loera, O., Guerrero-Olazarán, M., Viader-Salvadó, J. M., Gallegos-López, J. A., Fernández, F. J., … Viniegra-González, G. (2010). Cell growth and Trametes versicolor laccase production in transformed Pichia pastoris cultured by solid-state or submerged fermentations. Journal of Chemical Technology and Biotechnology, 85(4), 435–440. https://doi.org/10.1002/jctb.2306

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