Antiproliferative Activity of Allelochemicals Present in Aqueous Extract of Synedrella nodiflora (L.) Gaertn. In Apical Meristems and Wistar Rat Bone Marrow Cells

Abstract

Synedrella nodiflora (L.) Gaertn. is a kind of weed with ethno medicinal uses. Here, we aimed to evaluate the antiproliferative activity of AESN (aerial parts aqueous extract of S. nodiflora) in root and shoot apical meristems and WRBMCs (Wistar rat bone marrow cells). The phytotoxic and antiproliferative activities of AESN were evaluated using green-gram seedlings and onion roots. The AESN induced cell cycle delay was analysed by scoring mitotic index, interphase cell frequency, prophase-metaphase and anaphase-telophase cumulative frequency in onion apical meristem cells and by analysing metaphase frequency in WRBMCs. The AESN treatment showed dose dependent root and shoot growth retardation and reduced number of branch root sprouting in green-gram seedlings. Half maximal growth inhibitory concentration (IC 50) of AESN was 0.4 mg/ml at 48 h in onion. The mitotic index percentage significantly reduced (44.9 and 62.7% respectively for 0.5 and 2 mg/ml AESN cells at 48 h), interphase and prophase-metaphase cumulative frequency increased and anaphase-telophase cumulative frequency decreased in AESN treated onion root tip cells indicating an overall antiproliferative activity. In WRBMCs, AESN treatment could induce significant (p<0.001) mitodepression (80.6% decreased metaphase frequency with 500 mg/kg body weight for 15 h). These mitostatic activities of AESN may be due to the actions of allelochemicals. It may be said that the AESN contains antiproliferative active principle(s) that could induce significant delay in cell cycle kinetics in apical meristems and WRBMCs.