Objectives-Tooth eruption requires the presence of a dental follicle (DF), alveolar bone resorption for an eruption pathway, and alveolar bone formation at the base of the bony crypt. The objectivexs of our investigations have been to determine how the DF regulates both the osteoclastogenesis and osteogenesis needed for eruption. Material and Methods - Multiple experimental methods have been employed. Results - The DF regulates osteoclastogenesis and osteogenesis by regulating the expression of critical genes in both a chronological and spatial fashion. In the rat 1st mandibular molar there is a major burst of osteoclastogenesis at day 3 postnatally and a minor burst at day 10. At day 3, the DF maximally expresses colonystimulating factor-1 (CSF-1) to down-regulate the expression of osteoprotegerin (OPG) such that osteoclastogenesis can occur. At day 10, the minor burst of osteoclastogenesis is promoted by upregulation of vascular endothelial growth factor (VEGF) and RANKL in the DF. Spatially, the bone resorption is in the coronal portion of the bony crypt and genes such as RANKL are expressed more in the coronal region of the DF than in its basal one-half. For osteogenesis, bone formation begins at day 3 at the base of the bony crypt and maximal growth is at days 9-14. Osteo-inductive genes such as bone morphogenetic protein-2 (BMP-2) appear to promote this and are expressed more in the basal half of the DF than in the coronal. Conclusion - The osteoclastogenesis and osteogenesis needed for eruption are xregulated by differential gene expression in the DF both chronologically and spatially. © 2009 Blackwell Munksgaard.
CITATION STYLE
Wise, G. E. (2009, May). Cellular and molecular basis of tooth eruption. Orthodontics and Craniofacial Research. https://doi.org/10.1111/j.1601-6343.2009.01439.x
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