We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
CITATION STYLE
Paden, C. R., Tao, Y., Queen, K., Zhang, J., Li, Y., Uehara, A., & Tong, S. (2020). Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2. Emerging Infectious Diseases, 26(10), 2401–2405. https://doi.org/10.3201/eid2610.201800
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