Single molecule fluorescence polarization microscopy (smFPM) is a technique that enables to monitor changes in the orientation of a single labeled protein domain. Here we describe a smFPM microscope set-up and protocols to investigate conformational changes associated with the movement of motor proteins along cytoskeletal tracks.
CITATION STYLE
Benoit, M. P. M. H., & Sosa, H. (2018). Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin–microtubule complexes. In Methods in Molecular Biology (Vol. 1665, pp. 199–216). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7271-5_11
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