Ability to disrupt genes is essential in elucidating gene function. Unlike rodents or amphibians, it has been difficult to generate gene-targeted embryos in large animals. Therefore, studies of early embryo development have been hampered in large animals. A recent technology suggests that targeted mutations can be successfully introduced during embryogenesis, thus by-passing the need of breeding to produce gene-targeted embryos. This is particularly important in large animal models because of longer gestation period and higher animal cost. Here, we describe a specific approach to disrupt up to two genes simultaneously during embryogenesis using the CRISPR/Cas9 technology in swine. The approach can help understand the mechanism of zygotic genome activation in large animals.
CITATION STYLE
Ryu, J., & Lee, K. (2017). CRISPR/Cas9-mediated gene targeting during embryogenesis in swine. In Methods in Molecular Biology (Vol. 1605, pp. 231–244). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6988-3_16
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