Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.
CITATION STYLE
Bertaux-Skeirik, N., Centeno, J., Feng, R., Schumacher, M. A., Shivdasani, R. A., & Zavros, Y. (2016). Co-culture of gastric organoids and immortalized stomach mesenchymal cells. Methods in Molecular Biology, 1422, 23–31. https://doi.org/10.1007/978-1-4939-3603-8_3
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