We describe a direct method of measurement of free estradiol using equilibrium dialysis followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Serum aliquots and internal standards are extracted by liquid-liquid extraction using methyl-tert-butyl ether (MTBE) followed by derivatization with dansyl chloride. An API 5500 mass spectrometer operated in positive electrospray mode is used for detection.
CITATION STYLE
Ray, J. A., Kushnir, M. M., Rockwood, A. L., & Meikle, A. W. (2016). Direct measurement of free estradiol in human serum and plasma by equilibrium dialysis-liquid chromatography-tandem mass spectrometry. In Methods in Molecular Biology (Vol. 1378, pp. 99–108). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3182-8_12
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