Electroporation of non-viral plasmid DNA is a valuable tool to alter gene expression in the adult central nervous system. It offers a number of advantages over viral gene delivery as non-viral plasmids can integrate larger inserts and reduce the risk of inducing unintended immunological responses. Generally, electroporation of the adult brain is accomplished in rodents by applying high-Amplitude voltage-controlled pulses through the entire brain with plate electrodes surrounding the animal's head. Here, we describe an alternative electroporation protocol making use of current-controlled low-Amplitude pulses that are delivered locally by means of needlelike electrodes in the brain of adult mice. This allows altering gene expression in very-well-defined areas of the brain while inducing minimal tissue damage. © 2014 Springer Science+Business Media New York.
CITATION STYLE
De Vry, J., Vanmierlo, T., Martínez-Martínez, P., Losen, M., Temel, Y., Steinbusch, H., … Prickaerts, J. (2014). Delivery of DNA into the central nervous system via electroporation. Methods in Molecular Biology, 1121, 157–163. https://doi.org/10.1007/978-1-4614-9632-8_14
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