In Vitro DVS approach to evaluate skin reparation

Abstract

The stratum corneum (SC), the most superficial layer of the skin, is directly responsible for the skin's barrier function. The intercellular lipids in the SC play an important role in the regulation of the skin's water-holding capacity. The modification of the intercellular lipid organization and composition may impair these properties. The aim of the present study is to describe a new in vitro approach of the repairing capacity evaluation of lipid formulations on skin with the use of absorption and desorption curves. The formulations were applied on lipid-extracted SC, and the possible SC reparation was assessed with the use of a thermogravimetric balance (DVS). Moisture absorption/desorption experiments proved to be adequate for the evaluation of the repairing capacity of formulations applied on damaged skin. Besides, freeze-substitution transmission electron microscopy (FSTEM) images showed some lipid bilayers, indicating a lipid bilayer reconstitution due to the applied formulations.