Brotowali (Tinospora crispa L.) stem extract activity as an α-Amylase enzyme inhibitor

Abstract

Introduction: Reducing glucose absorption in the gastrointestinal tract is one of the strategies for treating diabetes mellitus. The condition of treating diabetes mellitus can be achieved by inhibiting the activity of the α-amylase enzyme. Brotowali (Tinospora crispa L.)/Tc has antihyperglycemic activity; compounds contained in the Tc stem can inhibit the activity of the α-amylase enzyme. The extraction of the Tc stem used for treatment was done with water and/or ethanol. Aim: This study aimed to measure the inhibitory activity of the α-amylase enzyme in both aqueous and ethanolic extract Tc stem. Methods: The inhibitory activity test of the α-amylase enzyme was carried out using the UV-visible spectrophotometric method. Results: The aqueous extract and ethanolic extract of Tc stem had α-amylase enzyme inhibitory activity with IC50 values of 11.660 ± 0.310 mg/mL and 10.348 ± 0.313 mg/mL, respectively. The Tc stem extracted with water or ethanol can be used as an antidiabetic drug.