Ce-mof with intrinsic haloperoxidase-like activity for ratiometric colorimetric detection of hydrogen peroxide

Abstract

Metal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of H2 O2 and Br−, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (Br4 PR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratio-metric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br−) system at wavelengths of 590 and 430, for quantifying H2 O2, where the detection limit of the H2 O2 is 3.25 µM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. More-over, through enzyme kinetics monitoring, the Km (H2 O2 and NH4 Br) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for H2 O2 and NH4 Br than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future.