Phage display is a biotechnique that fuses functional peptides on the outer surface of filamentous phage by inserting DNA encoding the peptides into the genes of its coat proteins. The resultant peptide-displayed phage particles have been widely used as biotemplates for the synthesis of functional hybrid nanomaterials. Here, we describe the bioengineering of M13 filamentous phage to surface-display bone mineral (hydroxyapatite (HAP))-nucleating peptides derived from dentin matrix protein-1 and using the engineered phage as a biotemplate to grow HAP nanocrystals. © 2014 Springer Science+Business Media, New York.
CITATION STYLE
Cao, B., Xu, H., & Mao, C. (2014). Phage as a template to grow bone mineral nanocrystals. Methods in Molecular Biology, 1108, 123–135. https://doi.org/10.1007/978-1-62703-751-8_10
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