HLA A∗32 is associated to HIV acquisition while B∗44 and B∗53 are associated with protection against HIV acquisition in perinatally exposed infants

Abstract

Background: Human leukocyte antigen (HLA) molecules play a key role in the cellular immune system. They may be determinants of mother-to-child transmission which is the driving force in pediatric HIV infection. We intended to look at the impact of the distribution of these polymorphic HLA genes in the mother-to-child transmission (MTCT) of HIV in Cameroon. Methods: A total of 156 mother-baby pairs were enrolled in three hospitals of Yaounde, capital of Cameroon. After the extraction of the DNA from blood samples using the Qiagen Kit as per manufacturer' instructions, the polymorphism of the HLA class 1 ABC was determined using the PCR- sequence specific primers assay. Results: The distribution of HLA class 1 revealed that none of the allele studied was associated with transmitters or non-transmitters, so was not implicated in transmission. The regression analysis showed that HLA A∗32 [OR 0.062 (CI; 0.0075 to 0.51)] is associated with HIV acquisition while HLA B∗44 [OR 0.47 (CI; 0.21 to 1.14)] and HLA B∗53 [OR; 0.14 (CI; 0.018 to 1.22)] were implicated in reducing the acquisition of HIV by infants. The homozygosity of locus C [OR 6.99 (CI; 1.81 to 26.88), p = 0.0027] was found as a risk factor for the acquisition, while the A∗32-B∗44 haplotype [OR 10.1 (CI 1.17 to 87.87), p = 0.03] was a risk factor for the transmission. Conclusion: This study has found that HLA A∗32, B∗44 and B∗53 have an impact in MTCT outcomes. The homozygosity of locus C and the A∗32-B∗44 haplotype were risk factors for acquisition and transmission respectively.