Can Oryzias Celebensis Embryo be Transported Dry?

Abstract

Embryos of the genus Oryzias have long been used as sentinel organisms in ecotoxicological research. Compared with animal models from mammals, Oryzias embryo offers several advantages such as being cost-effective, more sensitive, rapid and produce very little waste. In ecotoxicological studies, it is necessary to have inter-laboratory calibration on used techniques between one laboratory and another, so that the used techniques are reliable. Inter-laboratory calibration between laboratories requires transferring embryos from one laboratory to another. For this purpose, research has been carried out to compare the survival of embryos reared in water and non-water (dry) media until they hatch. The results showed that the embryos reared with dry media hatched one day faster than those raised in water media. The dry-incubated embryo also had an average total length longer than those incubated with embryo rearing media (ERM). In this study, it was concluded that fish embryos of Oryzias celebensis could be transported dry for up to five days.