Flow cytometry analysis and fluorescence-activated cell sorting (FACS) allow the determination and isolation of different cell types from a given tumor sample. Here we describe and comment a method consisting of the preparation of a single cell suspension from a freshly dissected mouse brain tumor mass, staining with a combination of fluorescently labeled antibodies and analysis by flow cytometry to determine, characterize, and isolate different immune populations.
CITATION STYLE
Godino, J., & Schuhmacher, A. J. (2019). Determination and isolation of immune populations from brain tumor microenvironments. In Methods in Molecular Biology (Vol. 1884, pp. 177–188). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8885-3_12
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