The yeast two-hybrid system is a useful tool for identifying new protein-protein interactions, and for the dissection of previously identified interactions. An important issue in protein-interaction studies is frequently that of determining whether a protein associates specifically with one protein or domain of interest, or has a more promiscuous interaction profile. To help address this issue, the authors have created a new two-hybrid system, which can be used either in bacteria or in yeast to counterscreen against "decoy" baits in parallel with a primary screen, hence improving the power and specificity of the method. Protocols of this system for use in yeast are provided; a companion article, Serebriiski et al., describes alternative use of this system in bacteria.
CITATION STYLE
Tikhmyanova, N. Y., Izumchenko, E. A., Serebriiskii, I. G., & Golemis, E. A. (2007). A bacterial/yeast merged two-hybrid system: protocol for yeast screening with single or parallel baits. Methods in Molecular Biology (Clifton, N.J.), 408, 257–290. https://doi.org/10.1007/978-1-59745-547-3_15
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