Microbiome research of host-associated communities has been advanced recently through improvements in sequencing technologies and bioinformatic methods. Traditional microbiological culture, when combined with molecular techniques, can provide a sensitive platform to comprehensively study the airway microbiota. Here we describe the culture methods necessary to capture a large proportion of the airway microbiota and molecular methods for profiling bacterial communities through the 16S rRNA gene, which, when combined, offer a more complete picture of the diversity of airway microbial communities than either method alone.
CITATION STYLE
Whelan, F. J., Rossi, L., Stearns, J. C., & Surette, M. G. (2018). Culture and Molecular Profiling of the Respiratory Tract Microbiota. In Methods in Molecular Biology (Vol. 1849, pp. 49–61). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8728-3_4
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