MicroRNA-335 is downregulated in bladder cancer and inhibits cell growth, migration and invasion via targeting ROCK1

Abstract

The expression of microRNA-335 (miR-335) has been demonstrated to be downregulated in numerous types of cancer. Thus far, no previous studies have investigated the MIR-335 expression in bladder cancer. In the present study, the expression and effects of MIR-335 were assessed in bladder cancer. The results of the present study provided, to the best of our knowledge, the first evidence that MIR-335 is downregulated in the tumor tissue of patients with bladder cancer. Following transfection of MIR-335, MTT, cell migration and invasion, luciferase and western blot assays were conducted in bladder cancer cell lines. The results demonstrated that MIR-335 inhibited cell proliferation, migration and invasion in T24 and EJ cells. In addition, the results suggested that MIR-335 directly targets Rho-associated protein kinase 1 (ROCK1) in bladder cancer. The present study provided a novel therapeutic target, the MIR-335/ROCK1 axis in bladder cancer. The suggested approach will be beneficial in developing an effective treatment against bladder cancer.