Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.
CITATION STYLE
Salone, V., & Rederstorff, M. (2015). Stem-Loop RT-PCR based quantification of small non-coding RNAs. Methods in Molecular Biology, 1296, 103–108. https://doi.org/10.1007/978-1-4939-2547-6_10
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