Optimization of media components for production of polyhydroxyalkanoates by Ralstonia eutropha using paddy straw as cheap substrate

Abstract

To reduce the production cost of polyhydroxyalkanoates (PHAs), the feasibility of using a lignocellulosic substrate—paddy straw as carbon source was examined. The design of an optimum and cheap medium for high level production of PHAs by Ralstonia eutropha was attempted by using response surface methodology. Based on the Plackett Burman design, K2HPO4 and MgSO4 were selected as the most critical mineral salts. Subsequently the optimum combination of the selected factors and the major carbon source, hydrolysed paddy straw were investigated by the Box–Behnken design.