Intracellular mechanisms regulating corticotropin-releasing hormone receptor-2β endocytosis and interaction with extracellularly regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling cascades

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Abstract

Many important physiological roles of the urocortin (UCN) family of peptides as well as CRH involve the type 2 CRH receptor (CRH-R2) and downstream activation of multiple pathways. To characterize molecular determinants of CRH-R2 functional activity, we used HEK293 cells overexpressing recombinant CRH-R2β and investigated mechanisms involved in attenuation of CRH-R2 signaling activity and uncoupling from intracellular effectors. CRH-R2β-mediated adenylyl cyclase activation was sensitive to homologous desensitization induced by pretreatment with either UCN-II or the weaker agonist CRH. CRH-R2β activation induced transient β-arrestin1 and β-arrestin2, as well as clathrin, recruitment to the plasma membrane. β-Arrestin2 appeared to be the main β-arrestin subtype associated with the receptor. This was followed by CRH-R2β endocytosis in a mechanism that exhibited distinct agonist-dependent temporal characteristics. CRH-R2β also induced transient activation of the ERK1/2 and p38MAPK signaling cascades that peaked at 5 min and returned to basal within 20-30 min. Unlike p38MAPK, activated ERK1/2 was localized both in the cytoplasm and nucleus. Experiments employing inhibitors of receptor endocytosis showed that CRH-R2β-MAPK interaction does not require β-arrestin, clathrin, or receptor endocytosis. Site-directed mutagenesis studies on CRH-R2β C terminus showed that the amino acid cassette TAAV at the end of the C terminus is important for CRH-R2β signaling because loss of a potential phospho-acceptor site in mutant receptors containing deletion or Ala substitution of the cassette TAAV resulted in reduced ERK1/2 activation and accelerated receptor internalization. These findings provide new insights about the signaling mechanisms regulating CRH-R2β functional activity and determining its biological responses. Copyright © 2008 by The Endocrine Society.

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Markovic, D., Punn, A., Lehnert, H., & Grammatopoulos, D. K. (2008). Intracellular mechanisms regulating corticotropin-releasing hormone receptor-2β endocytosis and interaction with extracellularly regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling cascades. Molecular Endocrinology, 22(3), 689–706. https://doi.org/10.1210/me.2007-0136

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