Phylogenetic relationships in the genus Apis inferred from mitochondrial DNA sequence data

Abstract

The nucleotide sequence of the 5’end of the mitochondrial cytochrome-oxydase sub- unit II gene was obtained through direct sequencing of PCR (Polymerase Chain Reaction) product from 4 Apis species (mellifera, cerana, dorsata and florea) and Bombus lucorum (Apidae) and Xylo- copa violacea (Anthophoridae) as and parsimony methods. Three branches, dorsata, florea and cerana-mellifera diverged almost si- multaneously and cerana separated from mellifera later but not as outgroups. Phylogenetic trees were built using Neighbor-Joining early as generally thought.