Gelfoam® histoculture provides a valuable tool for experimental studies of normal and pathological tissue physiology. It allows us to understand cell-cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam® histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with HIV and maintain them in Gelfoam® histoculture at the liquid-air interface. The Gelfoam® histocultures with human immunodeficiency virus (HIV) type 1-infected tissues have been used to further understand the biology of early HIV-1 pathogenesis, as well as a novel ex vivo platform to test the efficacy and toxicity of antiviral drugs.
CITATION STYLE
Introini, A., Fitzgerald, W., Vanpouille, C., & Margolis, L. (2018). Histoculture and infection with HIV of functional human lymphoid tissue on Gelfoam®. In Methods in Molecular Biology (Vol. 1760, pp. 187–197). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7745-1_17
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