Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam®, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge-matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to proper gene expression. The flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of av integrin, and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam®, the cells formed 3-dimensional tissue-like structures. These results indicate that Gelfoam® histoculture, unlike Matrigel™ culture, is true 3-dimensional.
CITATION STYLE
Tome, Y., Uehara, F., Kanaya, F., & Hoffman, R. M. (2018). Comparison of “Dimensionality” of cancer cell culture in Gelfoam® histoculture and matrigel. In Methods in Molecular Biology (Vol. 1760, pp. 205–214). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7745-1_19
Mendeley helps you to discover research relevant for your work.