Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 Stabilize the 43 S preinitiation complex

Abstract

Background: 43 S preinitiation complex (PIC) formation is an important step for eukaryotic translation initiation. Results: A thermodynamic framework of human 43 S PIC reveals a complicated network of positive and negative cooperativity among the components. Conclusion: eIF2 and eIF3 play essential roles in stabilizing the 43 S PIC. Significance: Quantitative analysis of initiation complex formation leads to understanding of mechanistic details in translation initiation.