Single-Cell Profiling of Fatty Acid Uptake Using Surface-Immobilized Dendrimers

Citations of this article
Mendeley users who have this article in their library.
Get full text


We present a chemical approach to profile fatty acid uptake in single cells. We use azide-modified analogues to probe the fatty acid influx and surface-immobilized dendrimers with dibenzocyclooctyne (DBCO) groups for detection. A competition between the fatty acid probes and BHQ2-azide quencher molecules generates fluorescence signals in a concentration-dependent manner. By integrating this method onto a microfluidics-based multiplex protein analysis platform, we resolved the relationships between fatty acid influx, oncogenic signaling activities, and cell proliferation in single glioblastoma cells. We found that p70S6K and 4EBP1 differentially correlated with fatty acid uptake. We validated that cotargeting p70S6K and fatty acid metabolism synergistically inhibited cell proliferation. Our work provided the first example of studying fatty acid metabolism in the context of protein signaling at single-cell resolution and generated new insights into cancer biology.




Guo, Z., Cheng, H., Li, Z., Shao, S., Sarkar, P., Wang, S., … Xue, M. (2021). Single-Cell Profiling of Fatty Acid Uptake Using Surface-Immobilized Dendrimers. Journal of the American Chemical Society, 143(29), 11191–11198.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free