Studies of the interaction of bacteria with mucus-secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far Western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed using biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high-throughput manner.
CITATION STYLE
Clyne, M., Duggan, G., Naughton, J., & Bourke, B. (2017). Methods to assess the direct interaction of C. jejuni with mucins. In Methods in Molecular Biology (Vol. 1512, pp. 107–115). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6536-6_10
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