Mucins are difficult to handle for their identification and characterization via proteomic applications due to their heavily glycosylated nature (up to 90%), high molecular weight (200 kDa-200 MDa), and size (Rg 10-300 nm). Their core proteins are extremely large and highly substituted with oligosaccharides, which only allow access to a highly restricted portion of their protein. For this reason, conventional 1D or 2D polyacrylamide gel-based proteomic approaches are not effective for identification and characterization of mucin molecules. In this chapter, we present our current protocol employing a modified shotgun proteomic approach to identify these complex glycoproteins. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Kesimer, M., & Sheehan, J. K. (2012). Mass spectrometric analysis of mucin core proteins. Methods in Molecular Biology, 842, 67–79. https://doi.org/10.1007/978-1-61779-513-8_4
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