Polysialic acid on neuropilin-2 is exclusively synthesized by the polysialyltransferase ST8SiaIV and attached to mucintype O-glycans located between the b2 and c domain

Abstract

Background: Polysialylated neuropilin-2 mediates CCL21-driven chemotactic migration of dendritic cells. Results: Deletion of either ST8SiaIV or O-glycosylation sites located between b2 and c domain abrogates polysialylation of neuropilin-2. Conclusion: Polysialylation of neuropilin-2 occurs in the same linker region as GAGylation of neuropilin-1. Significance: Defining enzyme and acceptor site requirements is crucial for understanding how polysialylation of neuropilin-2 is regulated. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.