Imaging techniques based on mass spectrometry (MS) have become powerful approaches to decipher the spatial distribution of metabolites and proteins. MS imaging (MSI) mostly relies on matrix-assisted laser desorption/ionization coupled to MS detection, but desorption electrospray ionization is also frequently used. Here we describe our current protocols for MALDI-MSI of seed sections and for root tissue. Detailed procedures for cryo-sectioning, matrix application, image capture, mass spectrometry measurement and data analysis are given. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Peukert, M., Becker, M., Matros, A., & Mock, H. P. (2014). Mass Spectrometry-based imaging of metabolites and proteins. Methods in Molecular Biology, 1072, 223–240. https://doi.org/10.1007/978-1-62703-631-3_17
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