Isolation and screening test of indigenous endophytic bacteria from areca nut rhizosphere as plant growth promoting bacteria

Abstract

The study aimed was to find out the endophytic bacteria isolated from areca nut rhizosphere in Southeast Sulawesi. The study was conducted at the Agronomy Laboratory, Faculty of Agriculture, Halu Oleo University. It using a completely randomized design (CRD) consist of four serial treatment of isolates isolated from 4 regencies. The isolates have tested their ability to improve viability and vigour of rice seed. The rice seeds before application, treated with endophytic bacteria isolates and then germinated. The germination rate was recorded daily by seven days. Each treatment using three replications, and data result were analysed using analysis of variance (ANOVA) and followed with DMRT (Duncan's Multiple Range Test). The result showed that the treatment of endophytic bacteria on rice seed has significantly increased the viability and vigour of rice seed. It also found four selected isolates which have potential to increase of rice seed growth, namely ME4, WSE4, WSE15, LE7, and LAE2. The future research is needed to find out the isolate that has effectivity on increase of areca nut seedling growth.