Design and implementation of the three-dimensional observation system for adult zebrafish

Abstract

In recent years, researchers have paid more attention to the neurobehavioral study of the adult zebrafish. It is very helpful to use a convenient observation system for the zebrafish experiments. However, the existing commercial observation systems are very expensive and the homemade systems are not flexible for different experiments. In this paper, we provide an observation system that has uniform illumination, multi-function, better flexibility and lower cost. Firstly, we designed a lighting system that has the uniform illumination through the optical simulation and polynomial fitting. Secondly, we designed the observation system, which includes tank modules and fixed bracket, so that the system can meet the requirements of many experiments. Finally, we chose white LEDs as light source and the aluminum profiles to implement the system, which make the system cheap and lightweight. In this paper, the observation system we designed achieves good result in the adult zebrafish experiments.