DTNB-based quantification of in vitro enzymatic N-terminal acetyltransferase activity

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Abstract

We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.

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Foyn, H., Thompson, P. R., & Arnesen, T. (2017). DTNB-based quantification of in vitro enzymatic N-terminal acetyltransferase activity. In Methods in Molecular Biology (Vol. 1574, pp. 9–15). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6850-3_2

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