D-Xylose concentration-dependent hydrolase expression profiles and the function of CreA and XlnR in Aspergillus niger

Abstract

Aspergillus niger is an important organism for the production of industrial enzymes such as hemicellulases and pectinases. The xylan-backbone monomer, D-xylose, is an inducing substance for the coordinate expression of a large number of polysaccharidedegrading enzymes. In this study, the responses of 22 genes to low (1 mM) and high (50 mM) D-xylose concentrations were investigated. These 22 genes encode enzymes that function as xylan backbone-degrading enzymes, accessory enzymes, cellulose-degrading enzymes, or enzymes involved in the pentose catabolic pathway in A. niger. Notably, genes encoding enzymes that have a similar function (e.g., xylan backbone degradation) respond in a similar manner to different concentrations of D-xylose. Although low D-xylose concentrations provoke the greatest change in transcript levels, in particular, for hemicellulase-encoding genes, transcript formation in the presence of high concentrations of D-xylose was also observed. Interestingly, a high D-xylose concentration is favorable for certain groups of genes. Furthermore, the repressing influence of CreA on the transcription and transcript levels of a subset of these genes was observed regardless of whether a low or high concentration of D-xylose was used. Interestingly, the decrease in transcript levels of certain genes on high D-xylose concentrations is not reflected by the transcript level of their activator, XlnR. Regardless of the D-xylose concentration applied and whether CreA was functional, xlnR was constitutively expressed at a low level. © 2012, American Society for Microbiology.