Isothermal titration calorimetry (ITC) is a powerful biophysical tool to characterize energetic profiles of biomacromolecular interactions without any alteration of the underlying chemical structures. In this protocol, we describe procedures for performing, analyzing, and interpreting ITC data obtained from a cooperative riboswitch–ligand interaction.
CITATION STYLE
Cavender, C. E., Schroeder, G. M., Mathews, D. H., & Wedekind, J. E. (2023). Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites Binding Model. In Methods in Molecular Biology (Vol. 2568, pp. 53–73). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2687-0_5
Mendeley helps you to discover research relevant for your work.