A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature

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Abstract

We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01-1.0 °C) using the new cationic fluorescent polymeric thermometer.

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Uchiyama, S., Tsuji, T., Ikado, K., Yoshida, A., Kawamoto, K., Hayashi, T., & Inada, N. (2015). A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature. Analyst, 140(13), 4498–4506. https://doi.org/10.1039/c5an00420a

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