Efficiency of cellulase production using coffee pulp waste under solid state fermentation by Aspergillus sp. VT12

Abstract

Activities in the production of coffee beans cause abundant coffee pulp wastes in many areas in Indonesia which can be an environmental problem. In contrast, this a huge agricultural waste can potentially be used through microbial utilization because of its cellulose polysaccharides content up to 63%. The fungus, known as cellulase producer Aspergillus sp. VT12 under solid-state fermentation (SSF) using coffee pulp based has been isolated. Investigation showed that optimum cellulase production by this isolate when SSF of 10 g coffee pulp, inoculated with 10s spores/ml, incubated at 30°C for 120 hours was done. Cellulase activity reached 1,8 U/ml based on reducing sugar released assayed at 37°C for 96 hours against 0,5% carboxymethyl cellulose (CMC) substrate in acetate buffer 20 mM pH 5. This cellulase stable at pH range i.e. pH 3-8 and optimum at pH 7. In this preliminary study of microbial utilisation of coffee pulp is a cheap-way to produce cellulase and may efficiently because without any nutrition either mineral added in the medium during production.