Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation can be applied to both native and reconstituted complexes. The preparations are characterized by their pigment composition, protein pattern, and spectral properties.
CITATION STYLE
Rühle, W., & Paulsen, H. (2011). Preparation of native and recombinant light-harvesting chlorophyll-a/b complex. Methods in Molecular Biology (Clifton, N.J.), 684, 113–125. https://doi.org/10.1007/978-1-60761-925-3_11
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