Calcium ion (Ca2+) is a ubiquitous intracellular messenger able to generate versatile intracellular signals that modulate a large variety of functions in virtually every cell type. Chemical and genetic biosensors, targeted to different subcellular compartments, have been developed and continuously improved to monitor Ca2+ dynamics in living cells. Here we describe the usage of Förster resonance energy transfer (FRET)-based Cameleon probes to investigate Ca2+ influx across the plasma membrane (PM) or Ca2+ release from the main intracellular Ca2+ store, the endoplasmic reticulum (ER).
CITATION STYLE
Galla, L., Pizzo, P., & Greotti, E. (2019). Exploiting cameleon probes to investigate organelles Ca2+ handling. In Methods in Molecular Biology (Vol. 1925, pp. 15–30). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9018-4_2
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