The polcalcin family is one of the most epidemiologically relevant families of calcium-binding allergens. Polcalcins are potent plant allergens that contain one or several EF-hand motifs and their allergenicity is primarily associated with the Ca2+-bound form of the protein. Conformation, stability, as well as IgE recognition of calcium-binding allergens greatly depend on the presence of protein-bound calcium ions. We describe a protocol that uses three techniques (SDS-PAGE, circular dichroism spectroscopy, and ELISA) to describe the effects that calcium has on the structural changes in an allergen and its IgE binding properties.
CITATION STYLE
Parody, N., Fuertes, M. Á., Alonso, C., & De Coaña, Y. P. (2013). Analysis of calcium-induced conformational changes in calcium-binding allergens and quantitative determination of their ige binding properties. In Methods in Molecular Biology (Vol. 963, pp. 115–125). Humana Press Inc. https://doi.org/10.1007/978-1-62703-230-8_8
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